Transcriptome Sequencing Analysis of Root in Soybean Responding to Mn Poisoning

Manganese (Mn) is among one of the essential trace elements for normal plant development; however, excessive Mn can cause growth and development to be hindered. Nevertheless, regulatory mechanisms root response poisoning remain unclear. In present study, results revealed that was inhibited when exposed poisoning. Physiological showed antioxidase enzyme activities (peroxidase, superoxide dismutase, ascorbate peroxidase, catalase) proline, malondialdehyde, soluble sugar contents increased significantly under toxicity stress (100 μM Mn), whereas protein four hormones’ (indolebutyric acid, abscisic indoleacetic gibberellic acid 3) decreased significantly. addition, Mn, Fe, Na, Al, Se in roots significantly, those Mg, Zn, K Furthermore, RNA sequencing (RNA-seq) analysis used test differentially expressed genes (DEGs) soybean The found 45,274 1430 DEGs concentrations 5 (normal) 100 (toxicity) μM. Among these DEGs, 572 were upregulated 858 downregulated, indicating may initiate complex molecular on stress. quantitative RT-PCR indicated many or downregulated markedly roots, suggesting regulation complex. Therefore, mechanism complicated. Present lay foundation further study function involved regulating tolerance traits roots.